de.mpg.escidoc.pubman.appbase.FacesBean
English
 
Help Guide Disclaimer Contact us Login
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Poster

Comparing Vero and MDCK cells for influenza A virus production in microcarrier systems

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons86303

Genzel,  Y.
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86276

Dietzsch,  C.
Dresden University of Technology,;
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;
Lehrstuhl für Bioverfahrenstechnik, Dresden;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86448

Reichl,  U.
Otto-von-Guericke-Universität Magdeburg;
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Genzel, Y., Dietzsch, C., & Reichl, U. (2007). Comparing Vero and MDCK cells for influenza A virus production in microcarrier systems. Poster presented at 20th ESACT Meeting, Dresden, Germany.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-97C5-3
Abstract
Each year the discussion on sufficient preparedness against an influenza pandemic is coming up. Several new approaches for effective vaccines are under investigation such as recombinant vaccines from baculovirus systems, virus like particles or designer cells as host cells. On the other hand, several processes using more established continuous cell lines are currently being licensed. Here, the favorite cell lines at the moment are: Vero and MDCK. These processes deal mostly with adherent cells, growing best on Cytodex microcarriers (GE Healthcare). Typically, data on cell growth and virus titers are found in literature. However, metabolism and cultivation in different bioreactors (stirred tank and wave) have not been discussed so far in a comparative study. Here, we present data for cultivations in roller bottles, 5L-stirred tank bioreactor as well as 2L-Wave bioreactors. Attachment properties in wave bioreactor conditions are discussed and cultivations with microcarrier concentrations of 2 g/L in serum containing and serum-free medium are compared. Differences between the two cell lines in metabolic data (glucose, lactate, glutamine, ammonia, glutamate) together with cell numbers and virus titers during cell growth and virus replication phase are analyzed to identify advantages and disadvantages of the presented cultivation conditions in respect to productivity.