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Abstract

Influenza viruses, major agents of respiratory diseases, are responsible for epidemics resulting in high mortality and morbidity every year. A better understanding of cellular virus-host cell interactions may help to explain the different mechanism of virulence and pathogenesis and may help to control or to prevent future outbreaks. The aim of this study was to characterize host cell protein expression changes in canine MDCK and human A549 cells after infection with human influenza A/PR/8/34 (H1N1). Host cell proteins from different infection phases were studied by twodimensional differential gel electrophoresis (2D-DIGE) and relative protein expression differences were quantified in order to gain a time-dependent proteomic coverage of host cell response. Differentially expressed proteins, with at least 2.5- fold expression changes and p values of 0.01 or less, were identified by mass spectrometry (LC-MS/MS). Here, we present proteins identified with these criteria. They are reported to be involved in a wide spectrum of cellular functions and host defense mechanisms including for example apoptosis, cytoskeletal rearrangement or protein synthesis and degradation. Distinct proteom patterns with different regulated proteins over time showed a dynamic host cell response mechanism in early and late phase of infection. Notably, the data showed that influenza A virus infection induced different marked alterations in protein expression in human or canine host cells. Surprisingly, also differences in host cell response were observed when comparing results from the same virus strain (H1N1) but from different suppliers (NIBSC, RKI).