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Glycan-analysis in influenza vaccine production based on cgelif: influence of host cell and virus on the glycosylationpattern of viral hemagglutinin

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons86442

Rapp,  Erdmann
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86475

Schwarzer,  Jana
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86321

Hennig,  René
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86303

Genzel,  Yvonne
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons86448

Reichl,  Udo
Otto-von-Guericke-Universität Magdeburg;
Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society;

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Citation

Rapp, E., Schwarzer, J., Hennig, R., Genzel, Y., & Reichl, U. (2009). Glycan-analysis in influenza vaccine production based on cgelif: influence of host cell and virus on the glycosylationpattern of viral hemagglutinin. Talk presented at EUROCARB 2009 (15th European Carbohydrate Symposium). Main University Vienna, Austria. 2009-07-19 - 2009-07-24.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0013-924C-C
Abstract
Mammalian cell culture processes, are commonly used for production of recombinant glycoproteins, antibodies and viral vaccines. Since several years there is an increasing interest in cell culture-based influenza vaccine production to overcome limitations of egg-based production systems, to improve vaccine supply and to increase flexibility in vaccine manufacturing. With the switch of the production system several key questions concerning the possible impact of host cell lines on antigen quality, passage-dependent selection of certain viral phenotypes or changes in hemagglutinin (HA) conformation have to be addressed to guarantee safety and efficiency of vaccines. Within this study, a capillary DNA-sequencer (based on CGE-LIF technology: capillary-gelelectrophoresis coupled online to laser-induced fluorescence detection) was utilized [1] for Nglycan analysis of different influenza virus strains, replicated in different mammalian cell lines. Detailed results concerning the influence of the host cell line on complexity and composition of the HA N-glycosylation pattern, are presented. Besides a strong host cell dependence of HA N-glycosylation that could be shown, a significant change in N-glycan type attached to HA was observed, comparing different virus types and subtypes [2]. [1] Schwarzer, J.; Rapp, E.; Reichl, U. Electrophoresis, 2008, 29, 4203-4214. [2] Schwarzer, J.; Rapp, E.; Hennig, R.; Genzel, Y.; Reichl U. Vaccine, 2008, submitted.