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Journal Article

An asymmetric SMC-kleisin bridge in prokaryotic condensin

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons101905

Bürmann,  Frank
Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons77713

Basquin,  Jerome
Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons101907

Giménez-Oya,  Victor
Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons78036

Gruber,  Stephan
Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Bürmann, F., Shin, H.-C., Basquin, J., Soh, Y.-M., Giménez-Oya, V., Kim, Y.-G., et al. (2013). An asymmetric SMC-kleisin bridge in prokaryotic condensin. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 20(3), 371-379. doi:10.1038/nsmb.2488.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000E-F62D-C
Abstract
Eukaryotic structural maintenance of chromosomes (SMC)-kleisin complexes form large, ring-shaped assemblies that promote accurate chromosome segregation. Their asymmetric structural core comprises SMC heterodimers that associate with both ends of a kleisin subunit. However, prokaryotic condensin Smc-ScpAB is composed of symmetric Smc homodimers associated with the kleisin ScpA in a postulated symmetrical manner. Here, we demonstrate that Smc molecules have two distinct binding sites for ScpA. The N terminus of ScpA binds the Smc coiled coil, whereas the C terminus binds the Smc ATPase domain. We show that in Bacillus sub fills cells, an Smc dimer is bridged by a single ScpAB to generate asymmetric tripartite rings analogous to eukaryotic SMC complexes. We define a molecular mechanism that ensures asymmetric assembly, and we conclude that the basic architecture of SMC-kleisin rings evolved before the emergence of eukaryotes.