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Book Chapter

Chromatin immunoprecipitation-based analysis of gene regulatory networks operative in human embryonic stem cells

MPS-Authors

Jung,  Marc
Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50054

Adjaye,  James
Molecular Embryology and Aging (James Adjaye), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Fulltext (public)

Jung.pdf
(Publisher version), 380KB

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Citation

Jung, M., & Adjaye, J. (2012). Chromatin immunoprecipitation-based analysis of gene regulatory networks operative in human embryonic stem cells. In Methods in molecular biology (pp. 269-80). New York: Springer.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000E-F057-9
Abstract
Chromatin immunoprecipitation (ChIP) followed by microarray-based (ChIP-Chip) or next-generation sequencing-based (ChIP-Seq) analysis has been established as a powerful and widely used method to investigate DNA-protein interactions relative to a genomic location in vivo. Here, we present a ChIP-Chip protocol, which utilizes an alternative, easier amplification protocol and when using high-quality ChIP-grade antibodies, will generate enough material for hybridization or sequencing with negligible enrichment bias due to amplification.