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Book Chapter

Immunoproteomics

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons81995

Krah,  Alexander
Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons81959

Jungblut,  Peter R.
Core Facilities / Proteinanalysis, Max Planck Institute for Infection Biology, Max Planck Society;

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Citation

Krah, A., & Jungblut, P. R. (2003). Immunoproteomics. In J. Decker (Ed.), Molecular diagnosis of infectious diseases (pp. 19-32). Totowa, N.J.: Humana.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000E-C5C9-7
Abstract
Two-dimensional electrophoresis results in an adequate resolution of the proteome of microorganisms to allow the detection and identification of specific antigens after blotting on membranes and overlaying the protein pattern with patient's sera. The complement of all identified antigens presents the immunoproteome of a microorganism. All the antigens specific for a microorganism or even for a disease are identified by mass spectrometry. For identification, peptide mass fingerprinting is used, and post-translational modifications are detected by mass spectrometry MS/MS techniques. High-resolution two-dimensional electrophoresis and unambiguous identification are prerequisites for reliable results. After statistical analysis, the resulting antigens are candidates for diagnosis or vaccination and targets for therapy.