de.mpg.escidoc.pubman.appbase.FacesBean
English
 
Help Guide Disclaimer Contact us Login
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons82193

Urban,  Johannes H.
Max-Planck Research Group RNA Biology, Max Planck Institute for Infection Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons82198

Vogel,  Jörg
Max-Planck Research Group RNA Biology, Max Planck Institute for Infection Biology, Max Planck Society;

Locator
There are no locators available
Fulltext (public)

PLoS_Biol_2008_6_e64.pdf
(Publisher version), 624KB

Supplementary Material (public)
There is no public supplementary material available
Citation

Urban, J. H., & Vogel, J. (2008). Two seemingly homologous noncoding RNAs act hierarchically to activate glmS mRNA translation. PLoS Biology, 6(3): e64, pp. 631-642.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000E-C1E5-9
Abstract
Small noncoding RNAs (sRNA) can function as posttranscriptional activators of gene expression to regulate stress responses and metabolism. We here describe the mechanisms by which two sRNAs, GlmY and GlmZ, activate the Escherichia coli glmS mRNA, coding for an essential enzyme in amino-sugar metabolism. The two sRNAs, although being highly similar in sequence and structure, act in a hierarchical manner. GlmZ, together with the RNA chaperone, Hfq, directly activates glmS mRNA translation by an anti-antisense mechanism. In contrast, GlmY acts upstream of GlmZ and positively regulates glmS by antagonizing GlmZ RNA inactivation. We also report the first example, to our knowledge, of mRNA expression being controlled by the poly(A) status of a chromosomally encoded sRNA. We show that in wild-type cells, GlmY RNA is unstable due to 3′ end polyadenylation; whereas in an E. coli pcnB mutant defective in RNA polyadenylation, GlmY is stabilized and accumulates, which in turn stabilizes GlmZ and causes GlmS overproduction. Our study reveals hierarchical action of two well-conserved sRNAs in a complex regulatory cascade that controls the glmS mRNA. Similar cascades of noncoding RNA regulators may operate in other organisms.