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Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection.

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons82148

Schuck,  Sebastian D.
Department of Immunology, Max Planck Institute for Infection Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons82064

Mueller,  Henrik
Department of Immunology, Max Planck Institute for Infection Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons81969

Kaufmann,  Stefan H. E.
Department of Immunology, Max Planck Institute for Infection Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons81950

Jacobsen,  Marc
Department of Immunology, Max Planck Institute for Infection Biology, Max Planck Society;

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PLoS_ONE_2009_4_e5590.pdf
(Verlagsversion), 896KB

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Zitation

Schuck, S. D., Mueller, H., Kunitz, F., Neher, A., Hoffmann, H., Franken, K. L. C. M., et al. (2009). Identification of T-cell antigens specific for latent mycobacterium tuberculosis infection. PLoS ONE, 4(5): e5590.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-000E-C0D8-4
Zusammenfassung
BACKGROUND: T-cell responses against dormancy-, resuscitation-, and reactivation-associated antigens of Mycobacterium tuberculosis are candidate biomarkers of latent infection in humans. METHODOLOGY/PRINCIPAL FINDINGS: We established an assay based on two rounds of in vitro restimulation and intracellular cytokine analysis that detects T-cell responses to antigens expressed during latent M. tuberculosis infection. Comparison between active pulmonary tuberculosis (TB) patients and healthy latently M. tuberculosis-infected donors (LTBI) revealed significantly higher T-cell responses against 7 of 35 tested M. tuberculosis latency-associated antigens in LTBI. Notably, T cells specific for Rv3407 were exclusively detected in LTBI but not in TB patients. The T-cell IFNgamma response against Rv3407 in individual donors was the most influential factor in discrimination analysis that classified TB patients and LTBI with 83% accuracy using cross-validation. Rv3407 peptide pool stimulations revealed distinct candidate epitopes in four LTBI. CONCLUSIONS: Our findings further support the hypothesis that the latency-associated antigens can be exploited as biomarkers for LTBI.