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Journal Article

Prohibitins Are Required for Cancer Cell Proliferation and Adhesion

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons82162

Sievers,  Claudia
Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons81815

Billig,  Gwendolyn
Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons81902

Gottschalk,  Kathleen
Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons82130

Rudel,  Thomas
Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society;

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Fulltext (public)

PLoS_ONE_2010_5_e12735.pdf
(Publisher version), 3MB

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Citation

Sievers, C., Billig, G., Gottschalk, K., & Rudel, T. (2010). Prohibitins Are Required for Cancer Cell Proliferation and Adhesion. PLoS ONE, 5(9): e12735.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000E-BF91-7
Abstract
Prohibitin 1 (PHB1) is a highly conserved protein that together with its homologue prohibitin 2 (PHB2) mainly localizes to the inner mitochondrial membrane. Although it was originally identified by its ability to inhibit G1/S progression in human fibroblasts, its role as tumor suppressor is debated. To determine the function of prohibitins in maintaining cell homeostasis, we generated cancer cell lines expressing prohibitin-directed shRNAs. We show that prohibitin proteins are necessary for the proliferation of cancer cells. Down-regulation of prohibitin expression drastically reduced the rate of cell division. Furthermore, mitochondrial morphology was not affected, but loss of prohibitins did lead to the degradation of the fusion protein OPA1 and, in certain cancer cell lines, to a reduced capability to exhibit anchorage-independent growth. These cancer cells also exhibited reduced adhesion to the extracellular matrix. Taken together, these observations suggest prohibitins play a crucial role in adhesion processes in the cell and thereby sustaining cancer cell propagation and survival.