English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
  Local TagsRelease HistoryDetailsSummary

Released
Journal Article

Cloned human and murine serotonin(3A) receptors expressed in human embryonic kidney 293 cells display different single- channel kinetics

MPS-Authors

Hapfelmeier,  G
Max Planck Institute of Psychiatry, Max Planck Society;

Haseneder,  R
Max Planck Institute of Psychiatry, Max Planck Society;

Lampadius,  K
Max Planck Institute of Psychiatry, Max Planck Society;

Rammes,  G
Max Planck Institute of Psychiatry, Max Planck Society;

Rupprecht,  R
Max Planck Institute of Psychiatry, Max Planck Society;

Zieglgänsberger,  W
Max Planck Institute of Psychiatry, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Hapfelmeier, G., Haseneder, R., Lampadius, K., Rammes, G., Rupprecht, R., & Zieglgänsberger, W. (2002). Cloned human and murine serotonin(3A) receptors expressed in human embryonic kidney 293 cells display different single- channel kinetics. Neuroscience Letters, 335(1), 44-48.


Cite as: https://hdl.handle.net/11858/00-001M-0000-000E-9FF9-1
Abstract
In the present study, well-resolved single-channel events of cloned human and murine homomeric 5-hydroxytryptamine type 3 (h5-HT3A and m5-HT3A) receptors, expressed in human embryonic kidney 293 cells, are reported for the first time. 5-HT (1 muM) applied in Ca2+- and Mg2+-free external solution to excised outside-out membrane patches induced non-rectifying single- channel inward currents. These currents were not observed in untransfected cells or in the presence of the antagonist ondansetron (1 muM). The mean single-channel conductance of the h5-HT3A and m5-HT3A receptors was 48 +/- 8 pS and 46 +/- 7 pS, the mean reversal potential was 10.3 +/- 1.8 mV and 4.7 +/- 5.3 mV, respectively. The analysis of single-channel open-times revealed for both h5-HT3A and m5-HT3A receptors only one type of open state, but different mean open-times (7.1 +/- 2.9 ms vs. 4.1 +/- 0.8 ms) indicating species-dependent gating mechanisms of 5-HT3 receptors. (C) 2002 Elsevier Science Ireland Ltd. All rights reserve