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Journal Article

Decoding Human Cytomegalovirus

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons78399

Michalski,  Annette
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons78085

Hein,  Marco Y.
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons78356

Mann,  Matthias
Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Stern-Ginossar, N., Weisburd, B., Michalski, A., Vu Khanh Le, T., Hein, M. Y., Huang, S.-X., et al. (2012). Decoding Human Cytomegalovirus. SCIENCE, 338(6110), 1088-1093. doi:10.1126/science.1227919.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000E-7A1B-E
Abstract
The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative transcript start sites plays a broad role in enabling tight temporal control of HCMV protein expression and allowing multiple distinct polypeptides to be generated from a single genomic locus. Our results reveal an unanticipated complexity to the HCMV coding capacity and illustrate the role of regulated changes in transcript start sites in generating this complexity.