How does tmRNA move through the ribosome?

Ivanov, Pavel V.; Zvereva, Maria I.; Shpanchenko, Olga V.; Dontsova, Olga A.; Bogdanov, Alexey A.; Aglyamova, Galina V.; Lim, Valery I.; Teraoka, Yoshika; Nierhaus, Knud H.

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How does tmRNA move through the ribosome?

MPG-Autoren

Ivanov, Pavel V.
Max Planck Society;

Zvereva, Maria I.
Max Planck Society;

Teraoka, Yoshika
Max Planck Society;

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Nierhaus, Knud H.
Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society;

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Zitation

Ivanov, P. V., Zvereva, M. I., Shpanchenko, O. V., Dontsova, O. A., Bogdanov, A. A., Aglyamova, G. V., et al. (2002). How does tmRNA move through the ribosome? FEBS Letters, 514(1), 55-59.

Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0010-8C6A-E

Zusammenfassung

To test the structure of tmRNA in solution, cross-linking experiments were performed which showed two sets of cross-links in two different domains of tmRNA. Site-directed mutagenesis was used to search for tmRNA nucleotide bases that might form a functional analogue of a codon–anticodon duplex to be recognized by the ribosomal A-site. We demonstrate that nucleotide residues U85 and A86 from tmRNA are significant for tmRNA function and propose that they are involved in formation of a tmRNA element playing a central role in A-site recognition. These data are discussed in the frame of a hypothetical model that suggests a general scheme for the interaction of tmRNA with the ribosome and explains how it moves through the ribosome.