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Wild-Type Levels of Spo11-Induced DSBs are Required for Normal Single-Strand Resection during Meiosis

MPG-Autoren

Trelles-Sticken,  Edgar
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50515

Scherthan,  Harry
Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Zitation

Neale, M. J., Ramachandran, M., Trelles-Sticken, E., Scherthan, H., & Goldman, A. S. H. (2002). Wild-Type Levels of Spo11-Induced DSBs are Required for Normal Single-Strand Resection during Meiosis. Molecular Cell, 9(4), 835-846.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-8C22-E
Zusammenfassung
We have studied the repair of a DNA-DSB created by the VMA1-derived endonuclease in mutants that have different levels of Spo11-DSBs: WT (sae2), few (hop1), and none (spo11-Y135F). In spo11-Y135F and hop1 cells, intrachromosomal repair is more frequent than in WT and sae2 cells. In spo11-Y135F cells there was no chromosome pairing or synapsis and a faster turnover of resected DNA. Compared to WT and sae2 cells, spo11-Y135F and hop1 cells have a greater proportion of long resection tracts. The data suggest that high levels of Spo11-DSBs are required for normal regulation of resection, even at a DSB created by another protein. WT control over resection could be important for directing repair to be interchromosomal, increasing the chance of creating interhomolog connections essential to meiotic segregation.