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High-throughput protein arrays: prospects for molecular diagnostics

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons50117

Büssow,  Konrad
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50167

Glökler,  Jörn
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Zitation

Walter, G., Büssow, K., Lueking, A., & Glökler, J. (2002). High-throughput protein arrays: prospects for molecular diagnostics. 6, 250-253.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-8BE8-F
Zusammenfassung
High-throughput protein arrays allow the miniaturized and parallel analysis of large numbers of diagnostic markers in complex samples. Using automated colony picking and gridding, cDNA or antibody libraries can be expressed and screened as clone arrays. Protein microarrays are constructed from recombinantly expressed, purified, and yet functional proteins, entailing a range of optimized expression systems. Antibody microarrays are becoming a robust format for expression profiling of whole genomes. Alternative systems, such as aptamer, PROfusion™, nano- and microfluidic arrays are all at proof-of-concept stage. Differential protein profiles have been used as molecular diagnostics for cancer and autoimmune diseases and might ultimately be applied to screening of high-risk and general populations. Abstract 2 : High-throughput protein arrays are still largely experimental but have taken the first steps towards becoming diagnostic tools, which will eventually arrive at the doctor's practice and as over-the-counter devices.