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Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis

MPG-Autoren

Giavalisco,  Patrick
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50448

Nordhoff,  Eckhard
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50409

Lehrach,  Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Gobom,  Joerg
Max Planck Society;

Klose,  Joachim
Max Planck Society;

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Zitation

Giavalisco, P., Nordhoff, E., Lehrach, H., Gobom, J., & Klose, J. (2003). Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis. Special Issue HPCE 2003, 207-216. doi:10.1002/elps.200390016.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-8AF9-2
Zusammenfassung
To increase the number of proteins detectable by two-dimensional electrophoresis (2-DE) in plants, we present a new procedure for extracting total proteins from plant tissue. This method avoids any loss of proteins in the course of sample preparation and results in two different fractions, one comprising mainly the cytoplasmatic proteins, the other one containing predominantly structure bond proteins. 2-DE patterns obtained from these two fractions show that the total number of different protein spots detected exceeds the degree of resolution commonly reported for plant proteins threefold.