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Sample preparation protocols for MALDI-MS of peptides and oligonucleotides using prestructured sample supports

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons50448

Nordhoff,  Eckhard
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50385

Kloeppel,  Klaus-Dieter
Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50409

Lehrach,  Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50168

Gobom,  Johan
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Zitation

Nordhoff, E., Schurenberg, M., Thiele, G., Luebbert, C., Kloeppel, K.-D., Theiss, D., et al. (2003). Sample preparation protocols for MALDI-MS of peptides and oligonucleotides using prestructured sample supports. International Journal of Mass Spectrometry, 226(1), 163-180. doi:10.1016/S1387-3806(02)00978-8.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-8A8A-9
Zusammenfassung
This contribution provides a set of protocols for MALDI-MS sample preparation of peptides and oligonucleotides on prestructured sample supports. The protocols have been optimized for high detection sensitivity, robust performance, ease of use, and include sample purification and concentration. Some protocols were optimized for manual preparation of individual samples. Others were developed for the use of automated pipetting stations and optimized for high throughput protein identification and DNA sequence analysis.