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Journal Article

Next generation of protein microarray support materials: Evaluation for protein and antibody microarray applications

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Angenendt,  Philipp
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Glökler,  Jörn
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Sobek,  Jens
Max Planck Society;

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Lehrach,  Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Cahill,  Dolores J.
Max Planck Society;

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Citation

Angenendt, P., Glökler, J., Sobek, J., Lehrach, H., & Cahill, D. J. (2003). Next generation of protein microarray support materials: Evaluation for protein and antibody microarray applications. Journal of Chromatography A, 1009(1-2), 97-104.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-89D0-2
Abstract
The performance of protein and antibody microarrays is dependent on various factors, one of which is the use of an appropriate microarray surface for the immobilisation of either protein or antibody samples. We have investigated the properties of seven new surfaces in the context of both protein and antibody microarray technology. We have demonstrated the functionality of all new slide coatings and investigated the mean signal to spotted concentration ratio, determined detection limits and calculated coefficients of variation. Moreover, new concepts for slide coatings such as dendrimer and poly(ethylene glycol)-epoxy slides were evaluated and improved qualities of novel slide surfaces were observed. Optimal slide coatings for antibody and protein chips were proposed and the requirements for both technologies were discussed.