Genome-wide RNAi analysis of growth and viability in Drosophila cells

Boutros, Michael; Kiger, Amy A.; Armknecht, Susan; Kerr, Kim; Hild, Marc; Koch, Britta; Haas, Stefan A.; Paro, Renato; Perrimon, Norbert

doi:10.1126/science.1091266

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Genome-wide RNAi analysis of growth and viability in Drosophila cells

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Haas, Stefan A.
Max Planck Society;

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Citation

Boutros, M., Kiger, A. A., Armknecht, S., Kerr, K., Hild, M., Koch, B., et al. (2004). Genome-wide RNAi analysis of growth and viability in Drosophila cells. Science, 303(5659), 832-835. doi:10.1126/science.1091266.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-88D7-B

Abstract

A crucial aim upon completion of whole genome sequences is the functional analysis of all predicted genes. We have applied a high-throughput RNA-interference (RNAi) screen of 19,470 double-stranded (ds) RNAs in cultured cells to characterize the function of nearly all (91%) predicted Drosophila genes in cell growth and viability. We found 438 dsRNAs that identified essential genes, among which 80% lacked mutant alleles. A quantitative assay of cell number was applied to identify genes of known and uncharacterized functions. In particular, we demonstrate a role for the homolog of a mammalian acute myeloid leukemia gene (AML1) in cell survival. Such a systematic screen for cell phenotypes, such as cell viability, can thus be effective in characterizing functionally related genes on a genome-wide scale.