de.mpg.escidoc.pubman.appbase.FacesBean
English
 
Help Guide Disclaimer Contact us Login
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

An enhancer directs differential expression of the linked Mrf4 and Myf5 myogenic regulatory genes in the mouse

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons50515

Scherthan,  Harry
Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Chang, T.-H.-T., Primig, M., Hadchouel, J., Tajbakhsh, S., Rocancourt, D., Fernandez, A., et al. (2004). An enhancer directs differential expression of the linked Mrf4 and Myf5 myogenic regulatory genes in the mouse. Developmental Biology, 269(2), 595-608. doi:10.1016/j.ydbio.2004.02.013.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-889B-4
Abstract
The myogenic regulatory factors, Mrf4 and Myf5, play a key role in skeletal muscle formation. An enhancer trap approach, devised to isolate positive-acting elements from a 200-kb YAC covering the mouse Mrf4–Myf5 locus in a C2 myoblast assay, yielded an enhancer, A17, which mapped at −8 kb 5′ of Mrf4 and −17 kb 5′ of Myf5. An E-box bound by complexes containing the USF transcription factor is critical for enhancer activity. In transgenic mice, A17 gave two distinct and mutually exclusive expression profiles before birth, which correspond to two phases of Mrf4 transcription. Linked to the Tk or Mrf4 minimal promoters, the nlacZ reporter was expressed either in embryonic myotomes, or later in fetal muscle, with the majority of Mrf4 lines showing embryonic expression. When linked to the Myf5 minimal promoter, only fetal muscle expression was detected. These observations identify A17 as a sequence that targets sites of myogenesis in vivo and raise questions about the mutually exclusive modes of expression and possible promoter/enhancer interactions at the Mrf4–Myf5 locus.