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A dedicated translation factor controls the synthesis of the global regulator Fis

MPG-Autoren

Owens,  Róisín M.
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50126

Connell,  Sean R.
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50444

Nierhaus,  Knud H.
Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society;

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Zitation

Owens, R. M., Pritchard, G., Skipp, P., Hodey, M., Connell, S. R., Nierhaus, K. H., et al. (2004). A dedicated translation factor controls the synthesis of the global regulator Fis. EMBO Journal, 23(16), 3375-3385. doi:10.1038/sj.emboj.7600343.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-87F3-4
Zusammenfassung
BipA is a highly conserved protein with global regulatory properties in Escherichia coli. We show here that it functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that is sensitive to high GDP:GTP ratios and stimulated by 70S ribosomes programmed with mRNA and aminoacylated tRNAs. The growth rate-dependent induction of BipA allows the efficient expression of Fis, thereby modulating a range of downstream processes, including DNA metabolism and type III secretion. We propose a model in which BipA destabilizes unusually strong interactions between the 5' untranslated region of fis mRNA and the ribosome. Since BipA spans phylogenetic domains, transcript-selective translational control for the ‘fast-track’ expression of specific mRNAs may have wider significance.