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A catalog of human cDNA expression clones and its application to structural genomics

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons50117

Büssow,  Konrad
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50476

Quedenau,  Claudia
Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50556

Sievert,  Volker
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Tischer,  Janett
Max Planck Society;

Scheich,  Christoph
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50550

Seitz,  Harald
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Hieke,  Brigitte
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50409

Lehrach,  Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Citation

Büssow, K., Quedenau, C., Sievert, V., Tischer, J., Scheich, C., Seitz, H., et al. (2004). A catalog of human cDNA expression clones and its application to structural genomics. Genome Biology, 5(9), R71-R71. doi:10.1186/gb-2004-5-9-r71.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-87EB-7
Abstract
We describe here a systematic approach to the identification of human proteins and protein fragments that can be expressed as soluble proteins in Escherichia coli. A cDNA expression library of 10,825 clones was screened by small-scale expression and purification and 2,746 clones were identified. Sequence and protein-expression data were entered into a public database. A set of 163 clones was selected for structural analysis and 17 proteins were prepared for crystallization, leading to three new structures.