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Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons50203

Himmelbauer,  Heinz
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Trachtulec et al - Biol J Linn Soc.pdf
(beliebiger Volltext), 124KB

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Zitation

Trachtulec, Z., Mihola, O., Vlcek, C., Himmelbauer, H., & Pacces, V. (2005). Positional cloning of the Hybrid sterility 1 gene: fine genetic mapping and evaluation of two candidate genes. Biological Journal of the Linnean Society, 84, 637-641. doi:10.1111/j.1095-8312.2005.00460.x.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-8762-C
Zusammenfassung
The Hybrid sterility 1 (Hst1) gene affects fertility of male hybrids between certain laboratory strains (such as C57BL/10) and some Mus musculus musculus mice by causing a breakdown of spermatogenesis at the stage of primary spermatocytes. In the process of positional cloning of the Hst1 gene, we generated a contig of bacterial artificial chromosomes and subsequently a low coverage sequence of the candidate region of the 129S1/SvImJ strain. Development of new genetic markers allowed us to narrow the Hst1 region from 580 to 360 kb. The products of two genes from this region, TATA-binding protein (Tbp) and proteasome subunit beta 1 (Psmb1), accumulate during spermatogenesis. These proteins have been described previously as having conserved C-terminal sequences and species-specific N-termini. We evaluated the candidacy of these genes for Hst1 by allelic sequencing and by real-time semiquantitative reverse-transcription PCR of testicular mRNAs.