de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Impressum Kontakt Einloggen
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling

MPG-Autoren

Gutjahr,  Claudia
Max Planck Society;

Lueking,  Angelika
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50388

Koenig,  Andrea
Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50363

Janitz,  Michal
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Horn,  Sabine
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50409

Lehrach,  Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)

Gutjahr et al. - Genomics.pdf
(beliebiger Volltext), 757KB

Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Gutjahr, C., Murphy, D., Lueking, A., Koenig, A., Janitz, M., O'Brien, J., et al. (2005). Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling. Genomics, 85(3), 285-296. doi:10.1016/j.ygeno.2004.11.005.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-8723-9
Zusammenfassung
The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (TH1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse TH1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays.