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Journal Article

Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling

MPS-Authors

Gutjahr,  Claudia
Max Planck Society;

Lueking,  Angelika
Max Planck Society;

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Koenig,  Andrea
Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Janitz,  Michal
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Horn,  Sabine
Max Planck Society;

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Lehrach,  Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Gutjahr et al. - Genomics.pdf
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Citation

Gutjahr, C., Murphy, D., Lueking, A., Koenig, A., Janitz, M., O'Brien, J., et al. (2005). Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling. Genomics, 85(3), 285-296. doi:10.1016/j.ygeno.2004.11.005.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-8723-9
Abstract
The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (TH1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particular clone to its recombinant, expressed protein. We have generated a mouse TH1 expression cDNA library and used protein arrays of this library to characterize the specificity and cross-reactivity of antibodies. Additionally, we have profiled the autoantibody repertoire in serum of a mouse model for systemic lupus erythematosus on these protein arrays and validated the putative autoantigens on highly sensitive protein microarrays.