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Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry

MPG-Autoren

Giavalisco,  Patrick
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50448

Nordhoff,  Eckhard
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Kreitler,  Thomas
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50385

Klöppel,  Klaus-Dieter
Mass Spectrometry (Head: David Meierhofer), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50409

Lehrach,  Hans
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Klose,  Joachim
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50168

Gobom,  Johan
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Volltexte (frei zugänglich)

Giavalisco et al. - Proteomics.pdf
(beliebiger Volltext), 713KB

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Zitation

Giavalisco, P., Nordhoff, E., Kreitler, T., Klöppel, K.-D., Lehrach, H., Klose, J., et al. (2005). Proteome analysis of Arabidopsis thaliana by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionisation-time of flight mass spectrometry. PROTEOMICS, 5(7), 1902-1913. doi:10.1002/pmic.200401062.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-8692-8
Zusammenfassung
In the present study we show results of a large-scale proteome analysis of the recently sequenced plant Arabidopsis thaliana. On the basis of a previously published sequential protein extraction protocol, we prepared protein extracts from eight different A. thaliana tissues (primary leaf, leaf, stem, silique, seedling, seed, root, and inflorescence) and analysed these by two-dimensional gel electrophoresis. A total of 6000 protein spots, from three of these tissues, namely primary leaf, silique and seedling, were excised and the contained proteins were analysed by matrix assisted laser desorption/ionisation time of flight mass spectrometry peptide mass fingerprinting. This resulted in the identification of the proteins contained in 2943 spots, which were found to be products of 663 different genes. In this report we present and discuss the methodological and biological results of our plant proteome analysis.