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Liquid-based hybridization assay with real-time detection in miniaturized array platforms

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons50180

Guerasimova,  Anna
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

Nyarsik,  Lajos
Max Planck Society;

Liu,  Jian-Ping
Max Planck Society;

Schwartz,  Regine
Max Planck Society;

Lange,  Matthias
Max Planck Society;

Lehrach,  Hnas
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50363

Janitz,  Michal
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Guerasimova.pdf
(beliebiger Volltext), 360KB

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Zitation

Guerasimova, A., Nyarsik, L., Liu, J.-P., Schwartz, R., Lange, M., Lehrach, H., et al. (2006). Liquid-based hybridization assay with real-time detection in miniaturized array platforms. Biomolecular Engineering, 23(1), 35-40. doi:10.1016/j.bioeng.2005.09.005.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-847E-6
Zusammenfassung
An assay for the fluorescent detection of short oligonucleotide probe hybridization in miniaturized high-density array platforms is presented. It combines hybridization in solution with real-time fluorescent detection, which involves measurement of fluorescence increase by means of an induced fluorescence resonance energy transfer. The feasibility of this approach using DNA or RNA as a target, and short DNA- as well as LNA (locked nucleic acid)-modified oligonucleotides as probes is shown. The presented approach could potentially contribute to a significant increase in the throughput of large-scale genomic applications, such as oligofingerprinting and genotyping, and also reduce material consumption.