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Characterization of Tau in cerebrospinal fluid using mass spectrometry

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons50448

Nordhoff,  Eckhard
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50168

Gobom,  Johan
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Zitation

Portelius, E., Hansson, S. F., Tran, A. J., Zetterberg, H., Grognet, P., Vanmechelen, E., et al. (2008). Characterization of Tau in cerebrospinal fluid using mass spectrometry. Journal of Proteome Reserach, 7(2), 2114-2120. doi:10.1021/pr7008669.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-7FF4-E
Zusammenfassung
The neurodegenerative disorder Alzheimer’s disease (AD) is the most common cause of dementia in the elderly. The presence of neurofibrillary tangles, consisting of hyperphosphorylated tau protein, is one of the major neuropathologic characteristics of the disease, making this protein an attractive biomarker for AD and a possible target for therapy. Here, we describe an optimized immunoprecipitation mass spectrometry method that enables, for the first time, detailed characterization of tau in human cerebrospinal fluid. The identities of putative tau fragments were confirmed using nanoflow liquid chromatography and tandem mass spectrometry. Nineteen tryptic fragments of tau were detected, of which 16 are found in all tau isoforms while 3 represented unique tau isoforms. These results pave the way for clinical CSF studies on the tauopathies.