de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Impressum Kontakt Einloggen
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Buchkapitel

In vitro selection of random RNA fragments to identify protein-binding sites within large RNAs

MPG-Autoren

Stelz,  Ulrich
Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50444

Nierhaus,  Knud
Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society;

Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Stelz, U., & Nierhaus, K. (2008). In vitro selection of random RNA fragments to identify protein-binding sites within large RNAs. In R.-J. Lin (Ed.), RNA-Protein Interaction Protocols (pp. 247-255). Berlin [et al]: Springer.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-7EED-A
Zusammenfassung
In vitro selection experiments have various goals depending on the composition of the initial pool and the selection method applied. We developed an in vitro selection variant that is useful for the identification of minimal RNA binding sites for proteins within large RNAs. A pool of randomly fragmented RNA is constructed from a large RNA, which is the natural binding partner for a protein. Such a pool contains all the potential binding sites and is therefore used as starting material for affinity selection. A successful in vitro selection with the purified protein will identify the protein's natural RNA target site. The method has been developed for ribosomal systems and is a general approach providing a basis for the functional and structural characterization of large ribonucleoprotein particles.