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Phylogenetic classification and identification of bacteria by mass spectrometry

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons50158

Freiwald,  Anja
Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons50512

Sauer,  Sascha
Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Citation

Freiwald, A., & Sauer, S. (2009). Phylogenetic classification and identification of bacteria by mass spectrometry. Nature Protocols, 4(5), 732-742. doi:10.1038/nprot.2009.37.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-7DAD-6
Abstract
Bacteria are a convenient source of intrinsic marker proteins, which can be detected efficiently by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The patterns of protein masses observed can be used for accurate classification and identification of bacteria. Key to the reliability of the method is a robust and standardized procedure for sample preparations, including bacterial culturing, chemical treatment for bacterial cell wall disruption and for protein extraction, and mass spectrometry analysis. The protocol is an excellent alternative to classical microbiological classification and identification procedures, requiring minimal sample preparation efforts and costs. Without cell culturing, the protocol takes in general <1 h.