A calibrated diversity assay for nucleic acid libraries using DiStRO-a 
Diversity Standard of Random Oligonucleotides

Schütze, T.; Arndt, P. F.; Menger, M.; Wochner, A.; Vingron, M.; Erdmann, V. A.; Lehrach, H.; Kaps, C.; Glökler, J.

doi:doi:10.1093/nar/gkp1108

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

A calibrated diversity assay for nucleic acid libraries using DiStRO-a Diversity Standard of Random Oligonucleotides

MPS-Authors

/persons/resource/persons50536

Schütze, T.
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

/persons/resource/persons50074

Arndt, P. F.
Evolutionary Genomics (Peter Arndt), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society;

/persons/resource/persons50613

Vingron, M.
Gene regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society;

Erdmann, V. A.
Max Planck Society;

/persons/resource/persons50409

Lehrach, H.
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

/persons/resource/persons50167

Glökler, J.
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

Schütze.pdf
(Any fulltext), 3MB

Supplementary Material (public)

There is no public supplementary material available

Citation

Schütze, T., Arndt, P. F., Menger, M., Wochner, A., Vingron, M., Erdmann, V. A., et al. (2010). A calibrated diversity assay for nucleic acid libraries using DiStRO-a Diversity Standard of Random Oligonucleotides. Nucleic Acids Research, 38(4), e23-e23. doi:doi:10.1093/nar/gkp1108.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-7BC8-5

Abstract

We have determined diversities exceeding 10(12) different sequences in an annealing and melting assay using synthetic randomized oligonucleotides as a standard. For such high diversities, the annealing kinetics differ from those observed for low diversities, favouring the remelting curve after annealing as the best indicator of complexity. Direct comparisons of nucleic acid pools obtained from an aptamer selection demonstrate that even highly complex populations can be evaluated by using DiStRO, without the need of complicated calculations.