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Pushing the detection limits: the evanescent field in surface plasmon resonance and analyte-induced folding observation of long human telomeric repeats

MPG-Autoren
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Konthur,  Z.
In vitro Ligand Screening (Zoltán Konthur), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Lehrach,  H.
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Glokler,  J.
Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society;

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Zitation

Schlachter, C., Lisdat, F., Frohme, M., Erdmann, V. A., Konthur, Z., Lehrach, H., et al. (2012). Pushing the detection limits: the evanescent field in surface plasmon resonance and analyte-induced folding observation of long human telomeric repeats. Biosensors & Bioelectronics, 31(1), 571-4. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/22152989 http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271399&_user=28761&_pii=S0956566311007470&_check=y&_origin=article&_zone=toolbar&_coverDate=15-Jan-2012&view=c&originContentFamily=serial&wchp=dGLbVBA-zSkzS&md5=a7375953639019aec0e47f3afe66e53d/1-s2.0-S0956566311007470-main.pdf http://pdn.sciencedirect.com/science?_ob=MiamiImageURL&_cid=271399&_user=28761&_pii=S0956566311007470&_check=y&_origin=article&_zone=toolbar&_coverDate=15-Jan-2012&view=c&originContentFamily=serial&wchp=dGLbVlV-zSkWz&md5=a7375953639019aec0e47f3afe66e53d/1-s2.0-S0956566311007470-main.pdf.


Zitierlink: https://hdl.handle.net/11858/00-001M-0000-0010-7765-5
Zusammenfassung
Conventional analysis of molecular interactions by surface plasmon resonance is achieved by the observation of optical density changes due to analyte binding to the ligand on the surface. Low molecular weight interaction partners are normally not detected. However, if a macromolecule such as DNA can extend beyond the evanescent field and analyte interaction results in a large-scale contraction, then the refractive index changes due to the increasing amount of macromolecules close to the surface. In our proof-of-principle experiment we could observe the direct folding of long, human telomeric repeats induced by the small analyte potassium using surface plasmon resonance spectroscopy. This work demonstrates the feasibility of new evanescent field-based biosensors that can specifically observe small molecule interactions.