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Protein Engineering of Stereoselective Baeyer–Villiger Monooxygenases

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons59138

Zhang,  Zhi-Gang
Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society;
Fachbereich Chemie, Philipps-Universität Marburgstitut für Kohlenforschung, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons58869

Parra,  Loreto P.
Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society;
Fachbereich Chemie, Philipps-Universität Marburgstitut für Kohlenforschung, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons58919

Reetz,  Manfred T.
Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society;
Fachbereich Chemie, Philipps-Universität Marburgstitut für Kohlenforschung, Max Planck Society;

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Citation

Zhang, Z.-G., Parra, L. P., & Reetz, M. T. (2012). Protein Engineering of Stereoselective Baeyer–Villiger Monooxygenases. Chemistry-a European Journal, 18, 10160-10172. doi:10.1002/chem.201202163.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-7544-A
Abstract
Baeyer–Villiger monooxygenases (BVMOs) have been used for decades as catalysts in stereoselective Baeyer–Villiger reactions, including oxidative kinetic resolution of racemic ketones and desymmetrization of prochiral substrates with high enantioselectivity. These complement catalytic BV processes based on chiral synthetic catalysts. However, as in any enzyme-catalyzed process, limitations exist due to the often observed narrow substrate scope and/or insufficient stereoselectivity. Recent protein engineering of BVMOs in the form of directed evolution and rational design have eliminated these traditional limitations, which is the subject of this Minireview. The main focus is on phenylacetone monooxygenase (PAMO); an unusually thermostable and robust BVMO, which has a very narrow substrate scope. Protein engineering of PAMO has provided a number of mutants that display relatively wide substrate scope, high stereoselectivity, and maintained thermostability.