English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Three-dimensional reconstruction of imperfect two-dimensional crystals

MPS-Authors
There are no MPG-Authors in the publication available
External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Saxton, W. O., Baumeister, W., & Hahn, M. (1984). Three-dimensional reconstruction of imperfect two-dimensional crystals. Ultramicroscopy, 13(1-2), 57-70.


Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-7538-6
Abstract
An outline is given of the general methodology of 3D reconstruction on the basis of correlation averages of the 2D projections: this hybrid real space/Fourier space approach substantially alleviates one of the most serious limitations on obtaining high resolution 3D structures, namely crystal distortions. The paper discusses some of the technical problems involved, namely optimisation of tilt increments, a posteriori tilt angle determination, extraction of lattice line data from averaged unit cells, and stain/protein boundary determination. The approach is illustrated by application to a 2D crystal from a bacterial cell envelope. (18 References).