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Journal Article

Three-dimensional structure of the tetragonal surface layer of Sporosarcina ureae

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Engelhardt, H., Saxton, W. O., & Baumeister, W. (1986). Three-dimensional structure of the tetragonal surface layer of Sporosarcina ureae. Journal of Bacteriology., 168(1), 309-317.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-74FE-3
Abstract
The three-dimensional structure of the regular surface layer of Sporosarcina ureae has been determined to a resolution of 1.7 nm by electron microscopy and image reconstruction. The S-layer has p4 symmetry, a lattice constant of 12.9 nm, and a minimum thickness of 6.6 nm. The reconstruction reveals a distinct domain structure: a massive core, arms connecting adjacent unit cells, and spurs which make contact at the subsidiary fourfold symmetry axes. In the z-direction the domains appear to be arranged in three planes, creating two entirely different surface reliefs. The S-layer has a complex pattern of pores and gaps that are 2 to 3 nm wide. In addition, the secondary-structure composition has been determined by infrared spectroscopy: about 35% of the polypeptide appears to have a beta-structure conformation.