Help Guide Disclaimer Contact us Login
  Advanced SearchBrowse




Journal Article

Three-dimensional structure of the tetragonal surface layer of Sporosarcina ureae

There are no MPG-Authors available
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available

Engelhardt, H., Saxton, W. O., & Baumeister, W. (1986). Three-dimensional structure of the tetragonal surface layer of Sporosarcina ureae. Journal of Bacteriology., 168(1), 309-317.

Cite as:
The three-dimensional structure of the regular surface layer of Sporosarcina ureae has been determined to a resolution of 1.7 nm by electron microscopy and image reconstruction. The S-layer has p4 symmetry, a lattice constant of 12.9 nm, and a minimum thickness of 6.6 nm. The reconstruction reveals a distinct domain structure: a massive core, arms connecting adjacent unit cells, and spurs which make contact at the subsidiary fourfold symmetry axes. In the z-direction the domains appear to be arranged in three planes, creating two entirely different surface reliefs. The S-layer has a complex pattern of pores and gaps that are 2 to 3 nm wide. In addition, the secondary-structure composition has been determined by infrared spectroscopy: about 35% of the polypeptide appears to have a beta-structure conformation.