Scanning tunnelling microscopy of biomacromolecules

Guckenberger, R.; Wiegräbe, W.; Baumeister, W.

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Scanning tunnelling microscopy of biomacromolecules

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Guckenberger, R., Wiegräbe, W., & Baumeister, W. (1988). Scanning tunnelling microscopy of biomacromolecules. Journal of Microscopy., 152(Pt 3), 795-802.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-74AE-6

Abstract

When imaging biomacromolecules with a STM, coating of specimens with a conductive layer is a convenient preparation method which provides a good rate of success. Utilizing evaporated platinum/carbon as a coating film we have investigated two biomacromolecules of very different appearance. The first of these is the HPI-layer, a natural two-dimensional protein crystal with a period of 18 nm, which is found on the surface of the bacterium Deinococcus radiodurans. The second specimen is type IV collagen which forms long triple-helical strands approx. 1.5 nm in diameter. The resulting STM pictures compare very well with electron microscopical images.