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Journal Article

Has negative staining still a place in biomacromolecular electron microscopy?

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Bremer, A., Henn, C., Engel, A., Baumeister, W., & Aebi, U. (1992). Has negative staining still a place in biomacromolecular electron microscopy? Ultramicroscopy., 46(1-4), 85-111.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-73F7-A
Abstract
Transmission electron microscopy of proteins has provided molecular- and in a few cases near-atomic-resolution structural information. In this review, we critically evaluate the potential and the limitations in obtaining molecular resolution, particularly with negatively stained specimens, and put these into perspective with cryomicroscopy of unstained frozen-hydrated and sugar-embedded preparations. [References: 114]