Localization of the Binding Site of an Antibody Affecting ATPase Activity of 
Chaperonin cpn60 from Bordetella pertussis

Cejka, Z.; Gould-Kostka, D.; Burns, D.; Kessel, M.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Localization of the Binding Site of an Antibody Affecting ATPase Activity of Chaperonin cpn60 from Bordetella pertussis

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Cejka, Z., Gould-Kostka, D., Burns, D., & Kessel, M. (1993). Localization of the Binding Site of an Antibody Affecting ATPase Activity of Chaperonin cpn60 from Bordetella pertussis. J. Struct. Biol., 111(1), 34-38.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-73C1-F

Abstract

Image processing has revealed the attachment site of antibody 54G8 on chaperonin 60 (cpn60) from Bordetella pertussis. This antibody, previously shown to affect the ability of chaperonin 10 (cpn10) to inhibit the ATPase activity of cpn60, is attached at the ends of the cpn60 and links the molecules into long chains. When only Fab fragments, which also affect ATPase activity, are used for labeling, these attach to both ends of the cpn60 molecule, but the long chains are not seen. Some perturbation of cpn60 was seen when Fab fragments were bound (Fab:cpn60 = 28:1).