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Journal Article

Cryo-electron microscopy of the surface protein of Sulfolobus shibatae

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Citation

Lembcke, G., Baumeister, W., Beckmann, E., & Zemlin, F. (1993). Cryo-electron microscopy of the surface protein of Sulfolobus shibatae. Ultramicroscopy, 49(1-4), 397-406.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-73B9-3
Abstract
Using cryo-microscopy at liquid-helium temperature the authors have determined the projection structure of the surface layer of the archaebacterium Sulfolobus shibatae embedded in aurothioglucose to a resolution of 0.85 nm. Image analysis was performed in real space and incorporated a classification of unit cells thus taking into account the frequent occurrence of twin boundaries. In an attempt to identify resolution-limiting factors they have performed a distortion analysis quantifying the main distortion parameters. (21 References).