de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Impressum Kontakt Einloggen
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Three-dimensional structure of lipid vesicles embedded in vitreous ice and investigated by automated electron tomography

MPG-Autoren
Es sind keine MPG-Autoren in der Publikation vorhanden
Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Dierksen, K., Typke, D., Hegerl, R., Walz, J., Sackmann, E., & Baumeister, W. (1995). Three-dimensional structure of lipid vesicles embedded in vitreous ice and investigated by automated electron tomography. Biophysical Journal, 68(4), 1416-1422.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-7341-2
Zusammenfassung
Automated electron tomography is shown to be a suitable means to Visualize the shape of phospholipid vesicles embedded in vitrified ice. With a slow-scan charge-coupled device camera as a recording device, the cumulative electron dose needed to record a data set of 60 projections at a magnification of 20,000x can be kept as low as 15 e(-)/Angstrom(2) (or 1500 electrons/nm(2)). The membrane of the three-dimensionally reconstructed vesicles is clearly Visible in two-dimensional sections through the three-dimensionally reconstructed volume. Some edges indicating a polygonal shape of the vesicles, frozen from the gel phase, are also clearly recognized. Because of the presently limited tilt angle range (+/-60 degrees), the upper and lower ''caps'' of the vesicles (representing about 35% of the surface of the ellipsoidal particles) remain invisible in the three-dimensional reconstruction. [References: 18]