Three-dimensional structure of lipid vesicles embedded in vitreous ice and 
investigated by automated electron tomography

Dierksen, K.; Typke, D.; Hegerl, R.; Walz, J.; Sackmann, E.; Baumeister, W.

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Three-dimensional structure of lipid vesicles embedded in vitreous ice and investigated by automated electron tomography

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Dierksen, K., Typke, D., Hegerl, R., Walz, J., Sackmann, E., & Baumeister, W. (1995). Three-dimensional structure of lipid vesicles embedded in vitreous ice and investigated by automated electron tomography. Biophysical Journal, 68(4), 1416-1422.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-7341-2

Abstract

Automated electron tomography is shown to be a suitable means to Visualize the shape of phospholipid vesicles embedded in vitrified ice. With a slow-scan charge-coupled device camera as a recording device, the cumulative electron dose needed to record a data set of 60 projections at a magnification of 20,000x can be kept as low as 15 e(-)/Angstrom(2) (or 1500 electrons/nm(2)). The membrane of the three-dimensionally reconstructed vesicles is clearly Visible in two-dimensional sections through the three-dimensionally reconstructed volume. Some edges indicating a polygonal shape of the vesicles, frozen from the gel phase, are also clearly recognized. Because of the presently limited tilt angle range (+/-60 degrees), the upper and lower ''caps'' of the vesicles (representing about 35% of the surface of the ellipsoidal particles) remain invisible in the three-dimensional reconstruction. [References: 18]