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Journal Article

Projection structure of the surface layer of methanoplanus limicola at 10-angstrom resolution obtained by electron cryomicroscopy

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Cheong, G. W., Typke, D., & Baumeister, W. (1996). Projection structure of the surface layer of methanoplanus limicola at 10-angstrom resolution obtained by electron cryomicroscopy. Journal of Structural Biology, 117(2), 138-144.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-7313-A
Abstract
The projection structure of the surface protein layer of the archaeon Methanoplanus limicola was analysed by electron microscopy and correlation averaging techniques to a resolution of about 1 nm. For electron microscopy the 2D crystalline protein layer was embedded in aurothioglucose. in order to minimize beam damage, specimen drift, charging and other effects degrading the images, cryomicroscopy at -120 degrees C in combination with spot-scan illumination technique with spot sizes in the range from 200 to 1000 nm was used for recording the images. (C) 1996 Academic Press, Inc. [References: 16]