de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Impressum Kontakt Einloggen
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT

Freigegeben

Zeitschriftenartikel

Highly ordered two-dimensional crystals of photosystem i reaction center from synechococcus sp - functional and structural analyses

MPG-Autoren
Es sind keine MPG-Autoren in der Publikation vorhanden
Externe Ressourcen
Es sind keine Externen Ressourcen verfügbar
Volltexte (frei zugänglich)
Es sind keine frei zugänglichen Volltexte verfügbar
Ergänzendes Material (frei zugänglich)
Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar
Zitation

Karrasch, S., Typke, D., Walz, T., Miller, M., Tsiotis, G., & Engel, A. (1996). Highly ordered two-dimensional crystals of photosystem i reaction center from synechococcus sp - functional and structural analyses. Journal of Molecular Biology, 262(3), 336-348.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-0010-72D2-2
Zusammenfassung
The photosystem I reaction center complex from the thermophilic cyanobacterium Synechococcus sp. was isolated by Triton X-100 solubilization and fractional precipitation with polyethylene glycol. As shown by gel electrophoresis, the isolated complex was composed of the 83 kDa subunits A and B, and at least six other subunits with molecular mass below 20 kDa. Electron transfer from the primary electron donor P700 to the F-A/F-B centers was demonstrated by flash-induced absorption change of the isolated complex, while electron paramagnetic resonance (EPR) spectroscopy showed that the complex contained a full set of Fe-S clusters. Isolated complexes were reconstituted into two-dimensional crystals in the presence of phospholipids and different cations. The crystals were found to be active by flash-induced separation and EPR spectroscopy. Electron microscopy and digital image processing of negatively stained and frozen-hydrated specimens revealed orthorhombic crystals with unit cell dimensions a = 138 Angstrom, b = 145 Angstrom and p12(1) symmetry. A three-dimensional map was calculated for negatively stained crystals to 19 Angstrom resolution, whereas the projection map of frozen-hydrated crystals exhibited 8 Angstrom resolution. (C) 1996 Academic Press Limited [References: 41]