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Electron tomography of single ice-embedded macromolecules - three-dimensional alignment and classification

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Walz, J., Typke, D., Nitsch, M., Koster, A. J., Hegerl, R., & Baumeister, W. (1997). Electron tomography of single ice-embedded macromolecules - three-dimensional alignment and classification. Journal of Structural Biology, 120(3), 387-395.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-726E-8
Abstract
From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level. (C) 1997 Academic Press. [References: 24]