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Robotic high-throughput assay for isolating apoptosis-inducing genes

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons78031

Grimm,  S.
Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons78176

Kachel,  V.
Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society;

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Grimm, S., & Kachel, V. (2002). Robotic high-throughput assay for isolating apoptosis-inducing genes. BioTechniques, 32(3), 670-677.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-6F9C-A
Abstract
The need to assign functions to genes will increase in upcoming years because of the accumulation of sequence information from various organisms. Here we present a genetic screen for the systematic functional analysis of genes in mammalian cells. The screen is based on high-throughput, transient transfections of individual cDNAs by a robotic system. This construction uses a moveable pipetting head with a novel 96-channel parallel tube- pump array and an integrated de-lidding device. Because of the robot design, the processing speed can reach 2000 transfections/h. We demonstrate the suitability of the screen to isolate apoptosis-inducing genes as a first read-out. This is achieved in combination with co-transfected reporter plasmids to produce a novel and convenient assay for apoptosis induction.