Interaction of the developmental regulator SALL1 with UBE2I and SUMO-1

Netzer, C.; Bohlander, S. K.; Rieger, L.; Muller, S.; Kohlhase, J.

アイテム詳細

登録内容を編集ファイル形式で保存

一時保存へ追加

タグ情報を表示リリース履歴を表示詳細要約

公開

学術論文

Interaction of the developmental regulator SALL1 with UBE2I and SUMO-1

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

There are no locators available

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

フルテキスト (公開)

公開されているフルテキストはありません

付随資料 (公開)

There is no public supplementary material available

引用

Netzer, C., Bohlander, S. K., Rieger, L., Muller, S., & Kohlhase, J. (2002). Interaction of the developmental regulator SALL1 with UBE2I and SUMO-1. Biochemical and Biophysical Research Communications, 296(4), 870-876.

引用: https://hdl.handle.net/11858/00-001M-0000-0010-6E70-1

要旨

Mutations in the SALL1 gene on chromosome 16q12.1 cause Townes- Brocks syndrome (TBS). This autosomal dominantly inherited disorder is characterized by typical malformations of the thumbs, the ears, and the anus, and also commonly affects the kidneys and other organ systems. SALL1 has recently been shown to localize to chromocenters and other heterochromatin foci in murine fibroblasts and to interact with the telomere-repeat- binding factor TRF1/PIN2. Here, we show that the ubiquitin- conjugating enzyme 21 (UBE2I), the human homolog of S. cerevisiae UBC9, and the small ubiquitin-like modifier-1 (SUMO- 1) interact with SALL1 in the yeast two-hybrid system. The interaction of SALL1 and UBE21 was confirmed in a glutathione S-transferase (GST) pull-down experiment. In an in vitro assay, it could be demonstrated that SALL1 is covalently modified by at least two SUMO-1 molecules in the presence of UBA2/AOS1 and UBE21. Mutation of lysine 1086 of SALL1 to arginine abrogates SALL1 sumoylation, suggesting the presence of a polymeric SUMO- I chain in the wild type state. (C) 2002 Elsevier Science (USA). All rights reserved.