Assisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of 
spliceosomal UsnRNPs

Meister, G.; Fischer, U.

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Assisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of spliceosomal UsnRNPs

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Meister, G.
Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society;

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Fischer, U.
Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Meister, G., & Fischer, U. (2002). Assisted RNP assembly: SMN and PRMT5 complexes cooperate in the formation of spliceosomal UsnRNPs. EMBO Journal, 21(21), 5853-5863.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-6DF6-0

Abstract

Although spliceosomal Sm proteins can assemble spontaneously onto UsnRNA in vitro, this process requires assisting factors in vivo. SMN, the protein involved in spinal muscular atrophy, is part of a complex that contains the Sm proteins and serves as a critical factor for this reaction. Here, we have reconstituted the SMN-dependent assembly of UsnRNPs in vitro. We demonstrate that the SMN complex is necessary and sufficient for the assembly reaction. The PRMT5 complex, previously implicated in methylation and storage of Sm proteins, interacts with the SMN complex and enhances its activity in an ATP- dependent manner. These data uncover the SMN-PRMT5 complex as a functional entity that promotes the assisted assembly of spliceosomal UsnRNPs, and potentially other, RNA-protein complexes.