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Journal Article

Crystal structure of halophilic dodecin: A novel, dodecameric flavin binding protein from Halobacterium salinarum

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons77761

Bieger,  B.
Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons78468

Oesterhelt,  D.
Oesterhelt, Dieter / Membrane Biochemistry, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Bieger, B., Essen, L. O., & Oesterhelt, D. (2003). Crystal structure of halophilic dodecin: A novel, dodecameric flavin binding protein from Halobacterium salinarum. Structure, 11(4), 375-385.


Cite as: http://hdl.handle.net/11858/00-001M-0000-0010-6C41-E
Abstract
A novel, 68 amino acid long flavoprotein called dodecin has been discovered in the proteome of Halobacterium salinarum by inverse structural genomics. The 1.7 Angstrom crystal structure of this protein shows a dodecameric, hollow sphere-like arrangement of the protein subunits. Unlike other known flavoproteins, which bind only monomeric flavin cofactors, the structure of the dodecin oligomer comprises six riboflavin dimers. The dimerization of these riboflavins along the refaces is mediated by aromatic, antiparallel pi staggering of their isoalloxazine moieties. A unique aromatic tetrade is formed by further sandwiching of the riboflavin dimers between the indole groups of two symmetry-related Trp36s. So far, the dodecins represent the smallest known flavoproteins. Based on the structure and the wide spread occurrences in pathogenic and soil eubacteria, a function in flavin storage or protection against radical or oxygenic stress is suggested for the dodecins.