Transcription of the tandem array of ribosomal DNA in Drosophila melanogaster 
does not terminate ar any fixed point

Tautz, Diethard; Dover, Gabriel A.

doi:10.1002/j.1460-2075.1986.tb04356.x

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Transcription of the tandem array of ribosomal DNA in Drosophila melanogaster does not terminate ar any fixed point

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Tautz, D., & Dover, G. A. (1986). Transcription of the tandem array of ribosomal DNA in Drosophila melanogaster does not terminate ar any fixed point. The EMBO Journal, 5(6), 1267-1273. doi:10.1002/j.1460-2075.1986.tb04356.x.

Cite as: https://hdl.handle.net/11858/00-001M-0000-0010-0F43-6

Abstract

Transcription termination of the polymerase I transcribed rRNA genes is thought to occur at or closely behind the end of the 28S gene coding region. We show here that this is not the case for Drosophila melanogaster. Nuclease S1 mapping of total RNA and a nuclear ‘run-on’ assay reveal that there is no defined point of termination throughout the whole spacer separating the two genes. Since the rRNA genes in D. melanogaster are organized in tandem arrays, this implies that readthrough transcription of one unit can occur up to, and possibly through, the promoter of the next unit. Readthrough transcription might be a component of transcription enhancement in a tandem array of genes.