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Conference Paper

Community structure and dynamics of bacterioplankton as revealed by low-molecular-weight RNA analysis

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons56730

Höfle,  M. G.
Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Höfle, M. G. (1993). Community structure and dynamics of bacterioplankton as revealed by low-molecular-weight RNA analysis. In R. Guerrero, & C. Pedrós-Alió (Eds.), Trends in Microbial Ecology: proceedings of the sixth International Symposium on Microbial Ecology (pp. 391-395). Barcelona: Spanish Society for Microbiology.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000F-E422-2
Abstract
The community structure of bacterioplankton was examined by the direct analysis of low-molecular-weight (LMW) RNA (5S rRNA and tRNAs). This approach allows determination of the taxonomic structure of natural microbial communities to the species or genus level, without the inherent bias of all culture-dependent identification methods. Using high resolution electrophoresis of all low molecular RNAs, relative changes in the microbial community structure were monitored in a set of lake water mesocosm experiments. In these mesocosm experiments the impact of a large-scale release of test bacteria (Escherichia coli, Pseudomonas putida) and their culture medium on the indigenous bacterioplankton community was studied. The dynamics of the bacterioplankton community as determined by LMW RNA analysis indicated a very fast response (after 24h) to the nutrient addition but no significant effects were detected by the massive release of the test bacteria.