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Nitrous oxide producing heterotrophic bacteria from the water column of the central Baltic: abundance and molecular identification

MPG-Autoren
http://pubman.mpdl.mpg.de/cone/persons/resource/persons56611

Brettar,  Ingrid
Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons56730

Höfle,  Manfred G.
Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Zitation

Brettar, I., & Höfle, M. G. (1993). Nitrous oxide producing heterotrophic bacteria from the water column of the central Baltic: abundance and molecular identification. Marine Ecology Progress Series, 94(3), 253-265.


Zitierlink: http://hdl.handle.net/11858/00-001M-0000-000F-E3FB-4
Zusammenfassung
The water column of the Gotland Deep, a basin with anoxic deep water in the central Baltic, was investigated for its denitrifying microflora in comparison with various chemical, hydrographical and microbiological parameters including in situ denitrification rates. Abundance of denitrifying bacterid was determined by the MPN method using nutrient broth plus nitrate medium. As gas formation in inverted vials turned out to be a rather variable feature, N2O production was used to detect the presence of denitrifying bacteria. Using 4 different cultivation approaches, 77 N2O producing strains were isolated from the whole water column. Isolates were analyzed for their denitrifying capacity to form NO2-, N2O, and N2. Taxonomic identification of the strains was done by high resolution electrophoresis of their low-molecular-weight (LMW) RNA (5S rRNA and tRNA). The bulk of the isolated N2O producing strains (77 %) was identified by their LMW RNA pattern as Shewanella putrefaciens. All strains of this species produced N2O, and NO2- and about one third showed N2-formation. S. putrefaciens is considered the most abundant culturable denitrifier of the low oxygen water and the oxic-anoxic interface of the Gotland Deep.