de.mpg.escidoc.pubman.appbase.FacesBean
English
 
Help Guide Disclaimer Contact us Login
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

Sequence analysis of the MHC class II DRB alleles in Alpine chamois (Rupicapra r. rupicapra)

MPS-Authors
http://pubman.mpdl.mpg.de/cone/persons/resource/persons56904

Schaschl,  Helmut
Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

Locator
There are no locators available
Fulltext (public)
There are no public fulltexts available
Supplementary Material (public)
There is no public supplementary material available
Citation

Schaschl, H., Goodman, S. J., & Suchentrunk, F. (2004). Sequence analysis of the MHC class II DRB alleles in Alpine chamois (Rupicapra r. rupicapra). Developmental and Comparative Immunology, 28(3), 265-277. doi:10.1016/j.dci.2003.08.003.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000F-DB14-B
Abstract
This study characterises the expressed MHC class 11 DRB gene and its genetic variation in exon 2 among 59 Alpine chamois (Rupicapra r. rupicapra) from several locations in the Eastern Alps plus one population in the Western Alps. The expressed DRB gene contains all the expected characteristics including all of the conserved residues found in other mammalian species. The isolated expressed DRB gene from chamois can be considered orthologous to MHC class 11 DRB genes in other mammalian species. Cattle primers yielded exon 2 sequences of 284 bp length. Sequence analysis revealed 19 DRB exon 2 alleles among 59 chamois. Alleles exhibited a high degree of nucleotide and amino acid polymorphism, with most amino acid variations occurring at positions forming the peptide-binding sites. A significantly higher rate of non-synonymous (dN = 0.053 ± 0.0132) than synonymous (dS = 0.007 ± 0.003) substitutions indicated positive selection for diversity in the DRB locus.