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Identification and characterization of 10 microsatellite primers for the calanoid freshwater copepods Eudiaptomus gracilis and E-graciloides using enriched genomic libraries

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http://pubman.mpdl.mpg.de/cone/persons/resource/persons57019

Zeller,  Martina
Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

http://pubman.mpdl.mpg.de/cone/persons/resource/persons56884

Reusch,  Thorsten B. H.
Department Ecophysiology, Max Planck Institute for Limnology, Max Planck Institute for Evolutionary Biology, Max Planck Society;
Department Evolutionary Ecology, Max Planck Institute for Evolutionary Biology, Max Planck Society;

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Citation

Zeller, M., & Reusch, T. B. H. (2004). Identification and characterization of 10 microsatellite primers for the calanoid freshwater copepods Eudiaptomus gracilis and E-graciloides using enriched genomic libraries. Molecular Ecology Notes, 4(3), 355-357. doi:10.1111/j.1471-8286.2004.00695.x.


Cite as: http://hdl.handle.net/11858/00-001M-0000-000F-DA87-F
Abstract
The calanoid copepods Eudiaptomus gracilis and Eudiaptomus graciloides are widespread among European lakes. We constructed enriched genomic libraries for both species in order to isolate and characterise microsatellite markers. The obtained 7 polymorphic microsatellite-loci for E. gracilis and 3 for E. graciloides are the first for any freshwater copepod. They display an expected heterozygosity ranging from 0.60 to 0.96 and 0.63 to 0.94 and observed heterozygosity ranging from 0.53 to 0.87 and 0.57 to 0.87, respectively. We were not able to cross-amplify the isolated loci across species, indicating long divergence among both congeneric species despite morphological similarity